Born in São Paulo (Brazil), Caroline De Comi received her Bachelor of Music degree from the University of São Paulo, where she studied with tenor Benito Maresca. She currently studies with Isabel Maresca. She has attended masterclasses by Tamás Salgó, Anna Korondi, Susan Bullock, Christina Landshamer, Peter Schreier, Luisa Castellani and Niza de Castro Tank.

Caroline has performed several roles on stage in Brazil, such as Gilda (Rigoletto), the Fire, the Princess and the Nightingale (L'enfant et les sortilèges), Queen of the Night (Die Zauberflöte), Madame Herz (Der Schauspieldirektor), Elisetta (Il Matrimonio Segreto), Norina (Don Pasquale) and Fiorilla (Il turco in Italia). In different opera productions, she worked with prominent stage directors in Brazil such as Livia Sabag, Mauro Wrona and Alvise Camozzi.

As a soloist in concert repertoire, she sang Pergolesi's Stabat Mater, Haydn's The Creation (as Eve), Mozart's Coronation Mass, Schubert's Mass in G, Fauré's Requiem, Saint-Säens's Christmas Oratorio, Händel's Messiah, and in the premieres of Mauricio De Bonis's Cantata para Caroline (which he dedicated to her) and Liduíno Pitombeira's Mariinha. She has performed in Brazil's major concert halls, as the Municipal Theater and the Sala São Paulo, and in the Brazilian Embassy in Asunción (Paraguay). She has performed as soloist wirh the  São Paulo Symphony Orchestra, the University of São Paulo Chamber Orchestra, the University of São Paulo Symphony Orchestra and the Americantiga group.

She sang in the 39th Música Nova Festival in São Paulo (under conductor Jack Fortner), and she was a finalist in the 8th Aldo Baldin Singing Competition. Among her recording are the DVD The Magic Flute, the Maestro and the Sorcerer (released by TUCCA, where she performed the Queen of the Night) and the CD Willy Corrêa de Oliveira, the present, with pianist Maurício De Bonis.